4EB2

Crystal structure Mistletoe Lectin I from Viscum album in complex with n-acetyl-d-glucosamine at 1.94 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-23
Detector	MAR CCD 165 mm
Wavelength(s)	0.81
Spacegroup name	P 65 2 2
Unit cell lengths	107.058, 107.058, 311.201
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.998 - 1.940
R-factor	0.1827
Rwork	0.180
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sz6
RMSD bond length	0.008
RMSD bond angle	1.011
Data reduction software	DENZO
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.500
High resolution limit [Å]	1.940
Rmerge	0.172
Number of reflections	77457
<I/σ(I)>	13.2
Completeness [%]	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.4	293	0.01 M sodium acetate, 25% saturated ammonium sulfate, 0.1 M glycin/HCl buffer, pH 3.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K