4EAF
Thymidine phosphorylase from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-13 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 129.951, 129.951, 67.809 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.000 - 1.550 |
| R-factor | 0.16031 |
| Rwork | 0.158 |
| R-free | 0.20686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tpt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.102 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.530 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.057 | 0.404 |
| Number of reflections | 86831 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 97.0 | 92.9 |
| Redundancy | 7.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 273 | COUNTER DIFFUSION, temperature 273K, LIQUID DIFFUSION |
