4EAE
The crystal structure of a functionally unknown protein from Listeria monocytogenes EGD-e
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97901 |
Spacegroup name | P 61 |
Unit cell lengths | 87.964, 87.964, 94.631 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.593 - 1.320 |
R-factor | 0.1592 |
Rwork | 0.158 |
R-free | 0.17730 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.111 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.000 | 1.350 |
High resolution limit [Å] | 1.320 | 1.320 |
Rmerge | 0.064 | 0.620 |
Number of reflections | 97196 | |
<I/σ(I)> | 43 | 3.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.4 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.1M DL-malic acid., pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |