4EAD
Thymidine phosphorylase from E.coli with 3'-azido-2'-fluoro-dideoxyuridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-09 |
| Detector | RAYONIX MX225HE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 129.862, 129.862, 67.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.000 - 1.500 |
| R-factor | 0.147 |
| Rwork | 0.145 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tpt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.443 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.510 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.062 | 0.519 |
| Number of reflections | 92521 | |
| <I/σ(I)> | 3.24 | |
| Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 273 | COUNTER DIFFUSION, LIQUID DIFFUSION, temperature 273K |
