4E9K
Crystal structure of a DUF4465 family protein (BACOVA_04221) from Bacteroides ovatus ATCC 8483 at 2.31 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91837,0.97975,0.97908 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 93.156, 93.156, 103.109 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.108 - 2.310 |
R-factor | 0.1798 |
Rwork | 0.178 |
R-free | 0.20380 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.606 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 29, 2011) |
Phasing software | SHELX |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.108 | 2.390 | |
High resolution limit [Å] | 2.310 | 4.950 | 2.310 |
Rmerge | 0.085 | 0.034 | 0.875 |
Number of reflections | 20538 | 2267 | 1968 |
<I/σ(I)> | 16.38 | 43.7 | 2.1 |
Completeness [%] | 99.8 | 99.6 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 2.4M ammonium sulfate, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |