4E9H
structure of glycosylase domain of MBD4 bound to 5hmU containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | .9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.090, 96.700, 55.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.870 - 3.000 |
R-factor | 0.1835 |
Rwork | 0.173 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.870 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 4698 | |
Completeness [%] | 99.2 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25% PEG 1500, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |