4E9F
Structure of the glycosylase domain of MBD4 bound to AP site containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.070, 55.840, 96.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.800 - 1.790 |
| R-factor | 0.2158 |
| Rwork | 0.214 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ene |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.063 | |
| Number of reflections | 21525 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.6 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 25% ethylene glycol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
