4E8X
Lambda-[Ru(TAP)2(dppz-(Me)2)]2+ bound to d(CCGGCGCCGG)2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 47.090, 47.090, 34.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.300 - 2.180 |
R-factor | 0.23521 |
Rwork | 0.233 |
R-free | 0.28370 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 2.694 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.090 | 2.240 |
High resolution limit [Å] | 2.180 | 2.180 |
Number of reflections | 2189 | |
Completeness [%] | 98.6 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1ul 2mM DNA, 1ul 2mM lambda-[Ru(TAP)2(dppz-(Me)2)]Cl2, 6ul 10% 2-methyl-2,4-pentanediol, 40mM sodium cacodylate, 12mM spermine tetra-HCl, 80mM potassium chloride, 20mM barium chloride, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |