4E8V
Structure of Oceanobacillus iheyensis group II intron in a ligand-free state in the presence of K+ and Ba2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.4850 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.727, 96.548, 227.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.908 - 3.995 |
| R-factor | 0.2992 |
| Rwork | 0.297 |
| R-free | 0.34550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3igi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.837 | 4.210 |
| High resolution limit [Å] | 3.990 | 3.990 |
| Rmerge | 0.069 | 0.787 |
| Number of reflections | 16781 | |
| <I/σ(I)> | 9.4 | 1.5 |
| Completeness [%] | 98.3 | 98.2 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 100 mM potassium acetate, 100 mM potassium chloride, 150 mM barium chloride, 50 mM HEPES sodium, pH 7.0, 6% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
