4E8U

Crystal structure of Arabidopsis IDN2 XS domain along with a small segment of adjacent coiled-coil region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	52.370, 207.022, 119.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.888 - 2.701
R-factor	0.254
Rwork	0.251
R-free	0.30580
Structure solution method	SAD (using a smaller construct) and MR
RMSD bond length	0.008
RMSD bond angle	1.175
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.087	0.045	0.547
Number of reflections	18040
<I/σ(I)>	11.2
Completeness [%]	99.0	97.3	96.3
Redundancy	10.3	9.6	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M BIS-TRIS pH 6.5, 2.0 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K