4E8N
Structure of Oceanobacillus iheyensis group II intron in a ligand-free state in the presence of NH4+ and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.889, 95.430, 224.554 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.100 - 2.960 |
R-factor | 0.19775 |
Rwork | 0.196 |
R-free | 0.23475 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3igi |
RMSD bond length | 0.009 |
RMSD bond angle | 2.720 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 3.040 |
High resolution limit [Å] | 2.960 | 2.960 |
Rmerge | 0.045 | 0.659 |
Number of reflections | 38193 | |
<I/σ(I)> | 15.9 | 1.7 |
Completeness [%] | 99.1 | 99.2 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 100 mM magnesium acetate, 200 mM ammonium chloride, 50 mM HEPES sodium, pH 7.0, 3% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 303K |