4E8M
Structure of Oceanobacillus iheyensis group II intron in a ligand-free state in the presence of K+ and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.170, 95.160, 224.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.450 - 3.500 |
| R-factor | 0.22794 |
| Rwork | 0.225 |
| R-free | 0.28020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3igi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.472 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.454 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.120 | 1.560 |
| Number of reflections | 22159 | |
| <I/σ(I)> | 10.1 | 1.5 |
| Completeness [%] | 97.6 | 95.6 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 100 mM magnesium acetate, 200 mM potassium chloride, 50 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 4% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
