4E8H
Structural of Bombyx mori glutathione transferase BmGSTD1 complex with GTT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.94968 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.109, 90.818, 87.015 |
| Unit cell angles | 90.00, 102.77, 90.00 |
Refinement procedure
| Resolution | 47.480 - 2.120 |
| R-factor | 0.19638 |
| Rwork | 0.195 |
| R-free | 0.23075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e8e |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.039 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.149 | 0.300 |
| Number of reflections | 48736 | |
| <I/σ(I)> | 13.3 | 4.6 |
| Completeness [%] | 95.5 | 95.5 |
| Redundancy | 6.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 8% pEG 8000, 0.1M sodium acetate trihydrate (pH 4.6), VAPOR DIFFUSION, HANGING DROP, temperature 289K |
