4E8B
Crystal structure of 16S rRNA Methyltransferase RsmE from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.479, 43.681, 74.883 |
| Unit cell angles | 90.00, 127.54, 90.00 |
Refinement procedure
| Resolution | 47.709 - 2.249 |
| R-factor | 0.2094 |
| Rwork | 0.207 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.246 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.249 | 2.250 |
| Rmerge | 0.042 | 0.209 |
| Number of reflections | 11818 | |
| <I/σ(I)> | 29.55 | 5.15 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M KSCN, 30%(w/v) PEG MME 2000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
