4E81
Crystal structure of the substrate binding domain of E.coli DnaK in complex with a short apidaecin peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 144.777, 60.202, 67.453 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.340 - 1.900 |
R-factor | 0.21072 |
Rwork | 0.208 |
R-free | 0.25942 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 2.116 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.340 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.530 | |
Number of reflections | 47233 | |
<I/σ(I)> | 2.8 | |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 2.7 M ammonium sulfate, 0.1 M MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |