4E7Y
Lambda-[Ru(phen)2(dppz)]2+ Bound to CCGGATCCGG
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 47.720, 47.720, 34.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.740 - 1.700 |
R-factor | 0.15683 |
Rwork | 0.155 |
R-free | 0.18837 |
Structure solution method | SAD |
RMSD bond length | 0.024 |
RMSD bond angle | 2.520 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.660 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 4747 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1ul 2mM DNA Oligomer, 1ul 3mM Lambda-[Ru(phen)2(dppz)]2+, 1ul 1mM Lambda-[Ru(tap)2(dppz-(Me)2]2+, 6ul 10% 1-methyl-2,4-pentanediol, 40mM Sodium cacodylate, 12mM Spermeine tetra-HCl, 80mM Potassium Chloride, 20mM Barium Chloride, equilibrated against 1ml 35% 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |