4E7W
Structure of GSK3 from Ustilago maydis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-06-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.982600 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 151.420, 151.420, 67.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.940 - 3.300 |
| R-factor | 0.2442 |
| Rwork | 0.241 |
| R-free | 0.29690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i09 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.400 | |
| High resolution limit [Å] | 3.300 | 15.000 | 3.300 |
| Rmerge | 0.060 | 0.019 | 0.665 |
| Number of reflections | 13635 | 153 | 1148 |
| <I/σ(I)> | 20.52 | 60.62 | 3 |
| Completeness [%] | 99.8 | 89.5 | 100 |
| Redundancy | 6.19 | 6.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 8-12% (w/v) EtOH, 1.5 M NaCl, 40 mM SrCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
