4E7U

The structure of T3R3 bovine insulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-28
Detector	MAR CCD 165 mm
Wavelength(s)	1.0391
Spacegroup name	H 3
Unit cell lengths	79.200, 79.200, 37.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.270 - 1.300
R-factor	0.1457
Rwork	0.144
R-free	0.17940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ben
RMSD bond length	0.008
RMSD bond angle	1.038
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 2010_07_29_2140))

Data quality characteristics

	Overall
Low resolution limit [Å]	21.270
High resolution limit [Å]	1.230
Number of reflections	23527
Completeness [%]	93.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH METHOD	6.4	298	6.5mg/ml bovine insulin, 7mM zinc acetate, 0.05M sodium citrate, 15% acetone, 0.2M KSCN, pH 6.4, BATCH METHOD, temperature 298K