4E7O

Crystal structure of receiver domain of putative NarL family response regulator spr1814 from Streptococcus pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 1 21 1
Unit cell lengths	52.868, 44.950, 59.304
Unit cell angles	90.00, 98.98, 90.00

Refinement procedure

Resolution	42.407 - 2.198
R-factor	0.2172
Rwork	0.214
R-free	0.27260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.084
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall
High resolution limit [Å]	2.198
Number of reflections	13021

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1M Tris-HCl, pH 8.0, 28% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 295K