4E7N
Crystal Structure of AhV_TL-I, a Glycosylated Snake-venom Thrombin-like Enzyme from Agkistrodon halys
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-03-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.91940 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.320, 68.360, 81.654 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.840 - 1.750 |
R-factor | 0.17936 |
Rwork | 0.178 |
R-free | 0.20519 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1op2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.355 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.448 | |
Number of reflections | 25563 | |
<I/σ(I)> | 5.17 | |
Completeness [%] | 99.8 | 98.7 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 287 | 22% PEG4000, 0.1M Tris, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 287K |