4E7J

PFV integrase Target Capture Complex, Apo form (TCC-Apo), at 3.15 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9763
Spacegroup name	P 41 21 2
Unit cell lengths	159.339, 159.339, 127.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.976 - 3.150
R-factor	0.2242
Rwork	0.222
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3os2
RMSD bond length	0.009
RMSD bond angle	1.443
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.320
High resolution limit [Å]	3.150	3.150
Rmerge	0.135	0.461
Number of reflections	28471
<I/σ(I)>	5.8	2
Completeness [%]	99.0	99.9
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	291	34% PEG-400, 180MM LI2SO4,2.5MM EDTA, 100MM TRIS-HCL, pH 7.4, VAPOR DIFFUSION, temperature 291K