4E74
Crystal structure of the RHO GTPASE BINDING DOMAIN of Plexin A4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 49.790, 49.790, 124.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.217 - 1.580 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r2o |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.471 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.217 | 1.620 | |
| High resolution limit [Å] | 1.580 | 7.070 | 1.580 |
| Rmerge | 0.052 | 0.029 | 0.986 |
| Number of reflections | 22346 | 324 | 1617 |
| <I/σ(I)> | 24.27 | 69.33 | 2.18 |
| Completeness [%] | 100.0 | 99.1 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% PEG3350, 0.2M ammonium acetate, 0.1M HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 291K |
