4E71

Crystal structure of the RHO GTPASE binding domain of Plexin B2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97934
Spacegroup name	P 65 2 2
Unit cell lengths	48.380, 48.380, 157.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.740 - 2.260
R-factor	0.2459
Rwork	0.244
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r2o
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.300
High resolution limit [Å]	2.260	6.130	2.260
Rmerge	0.098	0.064	0.999
Number of reflections	5712
<I/σ(I)>	8.8
Completeness [%]	99.7	96.1	100
Redundancy	19.6	14.2	21

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.6	291	2.5M sodium formate, 0.1M glycine, pH 9.6, vapor diffusion, sitting drop, temperature 291K