4E6U
Structure of LpxA from Acinetobacter baumannii at 1.4A resolution (P63 form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-06 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.979 |
Spacegroup name | P 63 |
Unit cell lengths | 75.751, 75.751, 119.063 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.090 - 1.410 |
R-factor | 0.17591 |
Rwork | 0.175 |
R-free | 0.19147 |
Structure solution method | 4E6T |
RMSD bond length | 0.010 |
RMSD bond angle | 1.291 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.460 |
High resolution limit [Å] | 1.410 | 1.410 |
Rmerge | 0.064 | 0.591 |
Number of reflections | 73881 | |
<I/σ(I)> | 36.1 | 5 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 11.3 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2ul of 25mg/ml protein, 2uL of 1.8M LiSO4, 0.1M Hepes 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |