4E6T
Structure of LpxA from Acinetobacter baumannii at 1.8A resolution (P212121 form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.696, 104.490, 106.979 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.610 - 1.800 |
R-factor | 0.1795 |
Rwork | 0.178 |
R-free | 0.21137 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qia |
RMSD bond length | 0.012 |
RMSD bond angle | 1.268 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.600 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.083 | 0.372 |
Number of reflections | 72800 | |
<I/σ(I)> | 12.1 | 3 |
Completeness [%] | 97.2 | 86.4 |
Redundancy | 5 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2ul of 15mg/ml protein, 2ul of 0.2M Ammonium citrate tribasic, 20% PEG 3350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |