4E6H
CRYSTAL STRUCTURE OF THE HAT domain of k. lactis RNA14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-18 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.350, 59.790, 123.480 |
| Unit cell angles | 90.00, 101.55, 90.00 |
Refinement procedure
| Resolution | 29.320 - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.27000 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB (THEN SOLVE/RESOLVE) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.053 | 0.247 |
| Number of reflections | 35927 | |
| <I/σ(I)> | 21.6721 | 4.207 |
| Completeness [%] | 93.9 | 79.4 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 50 mM HEPES 7.0, 1% tryptone, 13% PEG 3350, 5mm DTT, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
