4E6H
CRYSTAL STRUCTURE OF THE HAT domain of k. lactis RNA14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-18 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.350, 59.790, 123.480 |
Unit cell angles | 90.00, 101.55, 90.00 |
Refinement procedure
Resolution | 29.320 - 2.300 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.27000 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB (THEN SOLVE/RESOLVE) |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.053 | 0.247 |
Number of reflections | 35927 | |
<I/σ(I)> | 21.6721 | 4.207 |
Completeness [%] | 93.9 | 79.4 |
Redundancy | 4.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 50 mM HEPES 7.0, 1% tryptone, 13% PEG 3350, 5mm DTT, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |