4E6E

Crystal structure of a putative cell division protein FtsZ (Tfu_1113) from Thermobifida fusca YX-ER1 at 2.22 A resolution (PSI Community Target, van Wezel G.P.)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-08
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 32 2 1
Unit cell lengths	80.573, 80.573, 92.454
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.226 - 2.120
R-factor	0.1963
Rwork	0.195
R-free	0.23070
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE (December 29, 2011)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.286	40.290	2.180
High resolution limit [Å]	2.120	9.480	2.120
Rmerge	0.089	0.049	1.023
Total number of observations	224590
Number of reflections	20109	272	1444
<I/σ(I)>	19.3	55	2.2
Completeness [%]	99.8	99.1	99.8
Redundancy	11.17	11.4	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.600M magnesium sulfate, 0.1M MES pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K