4E6B
Crystal Structure of statistically disordered 19mer duplex p(CGG)3C(CUG)3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9724 |
| Spacegroup name | H 3 |
| Unit cell lengths | 39.779, 39.779, 34.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.470 |
| R-factor | 0.2048 |
| Rwork | 0.204 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.859 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.500 |
| High resolution limit [Å] | 1.470 | 3.990 | 1.470 |
| Rmerge | 0.049 | 0.045 | 0.169 |
| Number of reflections | 3488 | ||
| <I/σ(I)> | 24.1 | ||
| Completeness [%] | 99.4 | 100 | 97.1 |
| Redundancy | 3.4 | 11.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 1.9 mM ammonium sulfate, 5% isopropanol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
