4E5R
Crystal Structure of Frog DGCR8 Dimerization Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-01 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 39.891, 39.891, 82.125 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.882 - 1.900 |
| R-factor | 0.2088 |
| Rwork | 0.207 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.109 |
| Data reduction software | DENZO |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 5640 | |
| <I/σ(I)> | 1.23 | 3.38 |
| Completeness [%] | 99.0 | |
| Redundancy | 11.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20% (w/v) PEG-1000, 0.1 M imidazole pH 8.0, 0.2 M calcium acetate, 100 mM tribasic sodium citrate, vapor diffusion, hanging drop, temperature 277K |
