4E5M
Thermostable phosphite dehydrogenase E175A/A176R in complex with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.168, 114.013, 58.781 |
| Unit cell angles | 90.00, 94.11, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.850 |
| R-factor | 0.18733 |
| Rwork | 0.186 |
| R-free | 0.21688 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 12X phosphite dehydrogenase E175A |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.153 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.850 |
| Rmerge | 0.071 |
| Number of reflections | 51959 |
| <I/σ(I)> | 19.8 |
| Completeness [%] | 96.6 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 285 | 25-30% PEG 3350, 100 mM KCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
