4E53
Calmodulin and Nm peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.386, 79.285, 136.064 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.690 |
| R-factor | 0.2785 |
| Rwork | 0.276 |
| R-free | 0.31817 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.195 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 23563 | |
| Completeness [%] | 100.0 | 85.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.12mM MgAcO, 8% PEG 3350, 10% EtOH, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.12mM MgAcO, 8% PEG 3350, 10% EtOH, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
