4E52
Crystal structure of Haemophilus Eagan 4A polysaccharide bound human lung surfactant protein D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-24 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.350, 107.990, 55.650 |
| Unit cell angles | 90.00, 92.14, 90.00 |
Refinement procedure
| Resolution | 26.748 - 1.700 |
| Rwork | 0.192 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pw9 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.1.4) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.748 | 26.748 | 1.790 |
| High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
| Rmerge | 0.075 | 0.058 | 0.295 |
| Total number of observations | 6534 | 31047 | |
| Number of reflections | 71591 | ||
| <I/σ(I)> | 10.9 | 9.3 | 2.4 |
| Completeness [%] | 99.9 | 96.4 | 99.9 |
| Redundancy | 3 | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 4000, Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
