4E50
Calmodulin and Ng peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 |
Unit cell lengths | 76.467, 76.467, 46.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.24229 |
Rwork | 0.241 |
R-free | 0.27529 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.431 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 8503 | |
Completeness [%] | 100.0 | 92.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M imidazole, 1.2M sodium citrate tribasic dihydrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |