4E4Y
The crystal structure of a short chain dehydrogenase family protein from Francisella tularensis subsp. tularensis SCHU S4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97932 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 57.698, 79.260, 103.162 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.598 - 1.803 |
R-factor | 0.1642 |
Rwork | 0.162 |
R-free | 0.20360 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.024 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.079 | 0.472 |
Number of reflections | 21819 | |
<I/σ(I)> | 34.1 | 2.7 |
Completeness [%] | 98.6 | 100 |
Redundancy | 9.4 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1M Bis-Tris, 2.0M Ammonium sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |