4E4W
Structure of the C-terminal domain of the Saccharomyces cerevisiae MUTL alpha (MLH1/PMS1) heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.210, 66.140, 74.010 |
| Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
| Resolution | 39.790 - 2.500 |
| R-factor | 0.1722 |
| Rwork | 0.170 |
| R-free | 0.20990 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data scaling software | XSCALE |
| Phasing software | SHELXS |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 7.410 | 2.500 |
| Rmerge | 0.100 | 0.036 | 0.713 |
| Number of reflections | 31485 | ||
| <I/σ(I)> | 10.38 | 30.95 | 2.06 |
| Completeness [%] | 99.6 | 98.4 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
