4E4J
Crystal structure of arginine deiminase from Mycoplasma penetrans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.87260 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 120.548, 128.875, 220.279 |
Unit cell angles | 90.00, 91.41, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
Rwork | 0.210 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.325 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 290893 |
<I/σ(I)> | 9.8 |
Completeness [%] | 97.6 |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 1.7 M Ammonium sulfate, pH 7 bis-tris 0.1M amb 15% glyOH, VAPOR DIFFUSION, HANGING DROP |