4E4E
Crystal Structure of the Y34F mutant of Saccharomyces cerevisiae Manganese Superoxide Dismutase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9641 |
Spacegroup name | P 1 |
Unit cell lengths | 61.139, 63.022, 63.943 |
Unit cell angles | 112.19, 106.78, 103.88 |
Refinement procedure
Resolution | 32.183 - 1.880 |
R-factor | 0.1919 |
Rwork | 0.190 |
R-free | 0.23130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lsu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.149 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.970 |
High resolution limit [Å] | 1.880 | 4.090 | 1.880 |
Rmerge | 0.122 | 0.083 | 0.547 |
Number of reflections | 59948 | ||
<I/σ(I)> | 5.5 | ||
Completeness [%] | 96.7 | 93.6 | 96.3 |
Redundancy | 2.8 | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.2 M sodium malonate, 20% PEG 3350, pH 7.0, vapor diffusion, hanging drop, temperature 277K |