4E40

The haptoglobin-hemoglobin receptor of Trypanosoma congolense

Experimental procedure

Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.916
Spacegroup name	P 65 2 2
Unit cell lengths	104.530, 104.530, 113.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	90.530 - 1.600
R-factor	0.20677
Rwork	0.206
R-free	0.22332
Structure solution method	MIR
RMSD bond length	0.018
RMSD bond angle	1.818
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	90.530
High resolution limit [Å]	1.600
Number of reflections	46112
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	288	5% PEG 12,000, 0.1M sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K