4E3V
Crystal Structure of XYLOSE ISOMERASE FROM STREPTOMYCES RUBIGINOSUS Cryoprotected in Proline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-01-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.493, 98.022, 102.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.460 - 1.503 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.17930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xif |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.107 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.460 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 72416 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 2.0M ammonium sulfate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
