4E37
Crystal Structure of P. aeruginosa catalase, KatA tetramer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.733, 167.427, 90.547 |
Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
Resolution | 43.462 - 2.530 |
R-factor | 0.1757 |
Rwork | 0.174 |
R-free | 0.20050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m85 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.232 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_918)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.530 | 2.530 |
Number of reflections | 61328 | |
<I/σ(I)> | 15.58 | 5.15 |
Completeness [%] | 99.3 | 99 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 298 | 0.1 M sodium acetate, 16% PEG 3350, pH 4.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K |