4E2V
tRNA-guanine transglycosylase Y106F, C158V mutant in complex with preQ1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 113.15 |
| Detector technology | CCD |
| Collection date | 2010-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.363, 64.894, 70.375 |
| Unit cell angles | 90.00, 95.82, 90.00 |
Refinement procedure
| Resolution | 21.031 - 1.180 |
| R-factor | 0.1391 |
| Rwork | 0.138 |
| R-free | 0.15280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3GEV |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.202 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.200 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Number of reflections | 125858 | |
| <I/σ(I)> | 21.4 | 4.13 |
| Completeness [%] | 95.0 | 88 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 100 mM TRIS, 1 mM DTT, 10 % DMSO, 12 % PEG 8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
