4E2T
Crystal Structures of RadA intein from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97240 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.120, 67.370, 82.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.685 - 1.750 |
| R-factor | 0.1909 |
| Rwork | 0.190 |
| R-free | 0.23420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qlm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.645 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.700 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.953 |
| Number of reflections | 33297 | |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, 3.0 M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
