4E2S
Crystal structure of (S)-Ureidoglycine Aminohydrolase from Arabidopsis thaliana in complex with its substrate, (S)-Ureidoglycine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.23985 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.098, 174.894, 154.300 |
Unit cell angles | 90.00, 99.26, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.590 |
Rwork | 0.209 |
R-free | 0.27080 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.21) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.590 |
Number of reflections | 140969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES pH 7.5, 8% (w/v) PEG 8000, 8% (v/v) ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |