4E2K
The structure of the S. aureus DnaG RNA Polymerase Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.115 |
Spacegroup name | P 61 |
Unit cell lengths | 151.334, 151.334, 38.571 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.433 - 2.150 |
R-factor | 0.1921 |
Rwork | 0.191 |
R-free | 0.21860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dde |
RMSD bond length | 0.006 |
RMSD bond angle | 0.804 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 30.430 | 2.220 |
High resolution limit [Å] | 2.150 | 4.620 | 2.150 |
Rmerge | 0.072 | ||
Number of reflections | 28010 | ||
<I/σ(I)> | 30.5 | ||
Completeness [%] | 99.8 | 100 | 99 |
Redundancy | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |