4E2H

Crystal structure of the periplasmic domain of Shigella flexneri WzzB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Collection date	2011-07-11
Wavelength(s)	0.97912
Spacegroup name	P 1 21 1
Unit cell lengths	81.514, 62.619, 90.740
Unit cell angles	90.00, 94.67, 90.00

Refinement procedure

Resolution	49.597 - 2.360
R-factor	0.2483
Rwork	0.245
R-free	0.30250
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.394
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.700
High resolution limit [Å]	2.360
Number of reflections	22670
Completeness [%]	94.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20% PEG4000, 0.1M Hepes, 0.1M MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K