4E2E
Crystal structure of a tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG) from Homo sapiens at 2.25 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-07 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 60.988, 78.515, 122.131 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.685 - 2.250 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.22930 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 6, 2010) |
Phasing software | SOLVE |
Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.685 | 27.685 | 2.330 |
High resolution limit [Å] | 2.250 | 4.830 | 2.250 |
Rmerge | 0.061 | 0.030 | 0.904 |
Number of reflections | 14183 | 2684 | 2613 |
<I/σ(I)> | 11.83 | 29.3 | 1.5 |
Completeness [%] | 98.6 | 98.3 | 98 |
Redundancy | 6.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1.00M LiCl, 20.00% PEG-6000, 0.1M HEPES pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |