4E2C

Crystal Structure of the periplasmic domain of the chimeric LPS O-antigen chain length regulator protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-28
Wavelength(s)	0.97949
Spacegroup name	I 4 2 2
Unit cell lengths	116.946, 116.946, 219.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.500 - 2.800
Rwork	0.235
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.140
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.064	0.033	0.243
Number of reflections	23646
<I/σ(I)>	19.4
Completeness [%]	99.7	97.9	99.6
Redundancy	7.9	7.1	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	2.0M Sodium Citrate, 5% w/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K