4E29

Periplasmic domain of the chimeric WzzB chain length regulator protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.96406
Spacegroup name	I 4 2 2
Unit cell lengths	115.757, 115.757, 210.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.698 - 1.600
R-factor	0.2189
Rwork	0.218
R-free	0.23750
Structure solution method	AB INITIO PHASING
RMSD bond length	0.006
RMSD bond angle	0.923
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.698
High resolution limit [Å]	1.600
Number of reflections	90288

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	NaFormate, pH 6.0, VAPOR DIFFUSION, temperature 298K