4E1U
[Ru(bpy)2 dppz]2+ bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-12-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | .7749 |
Spacegroup name | P 1 |
Unit cell lengths | 24.039, 24.797, 37.521 |
Unit cell angles | 74.67, 84.42, 76.21 |
Refinement procedure
Resolution | 23.330 - 0.920 |
R-factor | 0.1401 |
Rwork | 0.140 |
R-free | 0.14900 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.620 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.000 |
High resolution limit [Å] | 0.920 |
Rmerge | 0.030 |
Number of reflections | 46920 |
<I/σ(I)> | 20 |
Completeness [%] | 82.7 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 296 | 20mM Cacodylate; 6mM Spermine*HCl; 40mM NaCl; 10mM BaCl2 5% 2-M-2,4PD, pH 7.0, VAPOR DIFFUSION, temperature 296K |