4E1Q

Crystal structure of Wheat Cyclophilin A at 1.25 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	197
Detector technology	CCD
Collection date	2012-02-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	37.135, 52.942, 77.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.855 - 1.251
R-factor	0.1778
Rwork	0.177
R-free	0.18500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.105
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	26.855
High resolution limit [Å]	1.251
Number of reflections	42895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	1.0M ammonium phosphate dibasic, 0.1M imidazole pH8.0, 0.2M NaCl , VAPOR DIFFUSION, SITTING DROP, temperature 291K